Section B: Molecular Characters
Characterization and quantification of genetic diversity have long been a major goal in species, sub-species and cultivar discrimination as well as plant breeding. Isozymes and random amplification of polymorphic DNA (RAPD) markers are powerful techniques for determining intra- and interspecific variations and allow direct comparison of plant variation at both biochemical and molecular levels (Williams et al., 1990; Welsh & McClelland, 1990; and Lu et al., 2009).
1. RAPD Diversity Aspects (Tabble 23)
In this study of genetic diversity in Caesalpinioid taxa with RAPD molecular technique, the level of DNA polymorphism detected with ten primers was very high and allowed the distinction of all taxa analysed. The high discriminatory power of the primers used indicates that the RAPD technique provides an effective tool for germplasm analysis in Caesalpinioideae.
All primers produced 323 bands and showed no monomorphic bands, meaning that the polymorphism investigated by these primers reached 100%.
Primer SC10-5 produced 17 polymorphic bands (14 common & three unique); SC10-14 showed 32 polymorphic bands, (eight unique); SC10-17 generated 24 polymorphic bands (seven unique); SC10-18 generated 46 polymorphic bands (ten unique); SC10-22 produced 22 polymorphic bands (12 bands unique); SC10-23 produced 47 polymorphic bands (nine unique); SC10-25 generated 32 polymorphic bands (11 unique); SC10-59 produced 31 polymorphic bands (13 unique); SC10-64 generated 39 polymorphic bands (nine unique) and SC11-30 showed 33 polymorphic bands (nine unique).
The data extracted from RAPD-PCR analysis for the studied taxa were amalgmated with the data from morphological and isozymes analysis then subjected to numerical analysis to interprete and discuss the interrelatioship between the taxa under investigation at generic and infra-specific level. Also, comparison of the schematic presentation with some of current systems of classification.
2. Isozyme Analysis (Table 24)
The data obtained from isozymes anlysis revealed highest degree of genetic diversity between the studied taxa of Caesalpinioideae. Acid phosphatase generated seven bands; alcohol dehydrogenase generated four bands; esterases generated 15 bands and aldehyde oxidase produced four bands.
All the enzyme systems analyzed were polymorphic where the inter-specific polymorphism reached 100%.
The data extracted from isozyme analysis for the studied taxa were amalgmated with the data from morphological and RAPD-PCR analysis then subjected to numerical analysis to interprete and discuss the interrelatioship between the taxa under investigation at generic and infra-specific level. Also, comparison of the schematic presentation with some of current systems of classification.
Characterization and quantification of genetic diversity have long been a major goal in species, sub-species and cultivar discrimination as well as plant breeding. Isozymes and random amplification of polymorphic DNA (RAPD) markers are powerful techniques for determining intra- and interspecific variations and allow direct comparison of plant variation at both biochemical and molecular levels (Williams et al., 1990; Welsh & McClelland, 1990; and Lu et al., 2009).
1. RAPD Diversity Aspects (Tabble 23)
In this study of genetic diversity in Caesalpinioid taxa with RAPD molecular technique, the level of DNA polymorphism detected with ten primers was very high and allowed the distinction of all taxa analysed. The high discriminatory power of the primers used indicates that the RAPD technique provides an effective tool for germplasm analysis in Caesalpinioideae.
All primers produced 323 bands and showed no monomorphic bands, meaning that the polymorphism investigated by these primers reached 100%.
Primer SC10-5 produced 17 polymorphic bands (14 common & three unique); SC10-14 showed 32 polymorphic bands, (eight unique); SC10-17 generated 24 polymorphic bands (seven unique); SC10-18 generated 46 polymorphic bands (ten unique); SC10-22 produced 22 polymorphic bands (12 bands unique); SC10-23 produced 47 polymorphic bands (nine unique); SC10-25 generated 32 polymorphic bands (11 unique); SC10-59 produced 31 polymorphic bands (13 unique); SC10-64 generated 39 polymorphic bands (nine unique) and SC11-30 showed 33 polymorphic bands (nine unique).
The data extracted from RAPD-PCR analysis for the studied taxa were amalgmated with the data from morphological and isozymes analysis then subjected to numerical analysis to interprete and discuss the interrelatioship between the taxa under investigation at generic and infra-specific level. Also, comparison of the schematic presentation with some of current systems of classification.
2. Isozyme Analysis (Table 24)
The data obtained from isozymes anlysis revealed highest degree of genetic diversity between the studied taxa of Caesalpinioideae. Acid phosphatase generated seven bands; alcohol dehydrogenase generated four bands; esterases generated 15 bands and aldehyde oxidase produced four bands.
All the enzyme systems analyzed were polymorphic where the inter-specific polymorphism reached 100%.
The data extracted from isozyme analysis for the studied taxa were amalgmated with the data from morphological and RAPD-PCR analysis then subjected to numerical analysis to interprete and discuss the interrelatioship between the taxa under investigation at generic and infra-specific level. Also, comparison of the schematic presentation with some of current systems of classification.
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